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 In order to assay the 17,20 lyase activity in the Δ5 pathway, the conversion of 17OHPREG to DHEA (Figure 3B) was assayed. FSH, follicle stimulating hormone; LH, luteinising hormone; DHEAS,dehydroepiandrostenedione-sulfate; SHBG, sex hormone binding globulin. FSH, follicle stimulating hormone; LH, luteinising hormone; DHEAS, dehydroepiandrostenedione-sulfate; SHBG, sex hormone binding globulin. No changes were observed in basal levels of T, E2, cortisol, cortisone, A4 (men only), 17OHPROG or aldosterone (Tables 1, 2).
 In the presence of both isoflavones, 17OHPREG and DHEA levels increased indicating the inhibition of 3βHSD2 activity, impacting steroid metabolism in the mineralocorticoid, glucocorticoid, and androgen pathways. The inhibition of the lyase activity of CYP17 by the isoflavones was investigated further in the H295R adrenal model to determine effects on the Δ5 pathway in the presence of competing enzymes viz 3βHSD2, SULT2A1, and 17βHSD. Neither daidzein nor genistein influenced levels of mineralocorticoids or glucocorticoids significantly in the study subjects. Both isoflavones inhibited the lyase activity significantly with daidzein exhibiting a greater inhibitory effect.
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